Gene CNs of FAM3C, MET, EGFR, and FGFR1 were calculated using the genome sequencing datasets of several cancer entities in the TCGA database (https://www.cancer.gov/about-nci/organization/ccg/research/structural-genomics/tcga). GISTIC 2.0 values were obtained from the Firehose system using Caleydo v3 software (caleydo.org): − 2, deep deletion; − 1, shallow deletion; 0, diploid; 1, gain; 2, amplification. RNA-seq data were obtained from Cancer Browser database (https://genome-cancer.ucsc.edu/).

Copy number (CN) data of 200 tumor cell lines were generated using the GeneChip Human Mapping 250 K Nsp Arrays (Affymetrix) and subsequently analyzed on the Affymetrix® Genotyping Console™ software (GTC) using the unpaired CNAT 4.0 analysis algotrythms. Gene copy number ± 3 was considered as amplification, < 3 as non-amplified.

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