All animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) and were consistent with the Public Health Service policy on Humane Care and Use of Laboratory Animals (Office of Laboratory Animal Welfare, 2002). The use of human samples was approved by the Institutional Review Board (IRB). Total RNA were extracted from whole lung homogenates of newborn C57BL/6 J mice using miRNeasy Mini Kit from Qiagen at three developmental time points [postnatal day 1 (P1), P14, and P42]. miR-219-5p and PDGFR-α mRNA were measured by RT-PCR. All experiments were done with a minimum of six mice from at least two litters for each condition.

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