Fundus images were obtained. Animals were anesthetized with an intraperitoneal injection of 60 mg/kg ketamine hydrochloride (Fort George Animal Centre, Southampton, UK) and 5 mg/kg xylazine (Pharmacia & Veterinary Products, Kiel, Germany). The pupils were dilated with a drop of tropicamide and phenylephrine (Chauvin, Essex, UK). Images were captured with a Nikon D90 camera via an endoscope [36, 37] or the Micron IV system (Phoenix Research Labs, Pleasanton, CA, USA). Retinal green fluorescent images in Cx3cr1gfp/gfp and DKO mice were acquired using the Micron IV system. The illumination settings and the gain were consistent in each subject. Images were saved in TIFF format.

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