For immunofluorescence, the cells seeded on the coverslips were washed with PBS and fixed with 4 % PFA, then treated with 0.1 % Triton X-100. After blocking with goat serum, the cells were treated with primary antibody and FITC-labeled or Cy3-labeled secondary antibody, respectively. The nuclei were labeled with DAPI and visualized by confocal microscope. For IHC, put the paraffin sections after deparaffinization in citrate buffer for antigen retrieval, and then blocked with 3 % H2O2. They were then incubated with primary and secondary antibodies, and finally with the DAB chromogen solution and HRP substrate solution. The primary antibodies were as follows: anti-Ki-67, 1:200 (Novus); anti-TEK, 1:200 (Abcam).

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