For immunofluorescence, the cells seeded on the coverslips were washed with PBS and fixed with 4 % PFA, then treated with 0.1 % Triton X-100. After blocking with goat serum, the cells were treated with primary antibody and FITC-labeled or Cy3-labeled secondary antibody, respectively. The nuclei were labeled with DAPI and visualized by confocal microscope. For IHC, put the paraffin sections after deparaffinization in citrate buffer for antigen retrieval, and then blocked with 3 % H2O2. They were then incubated with primary and secondary antibodies, and finally with the DAB chromogen solution and HRP substrate solution. The primary antibodies were as follows: anti-Ki-67, 1:200 (Novus); anti-TEK, 1:200 (Abcam).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.