The collected cells were lysed in a mixture of RIPA buffer (Sigma-Aldrich, USA), phosphatase inhibitors and protease inhibitors in the ratio of 50:1:1 for 30 minutes on ice. We used a 10 % SDS / PAGE gel to separate proteins and transfer them to a PVDF membrane (Millipore), then sealed them with 5 % skimmed milk and incubated with primary and secondary antibodies. The primary antibodies were as follows: anti-TEK, 1:1000 (Abcam); anti-GAPDH, 1:1000 (Santa Cruz); anti-N-cadherin, 1:500 (CST); anti-E-cadherin, 1:500 (CST); anti-Vimentin, 1:1000 (CST); anti-β-catenin, 1:1000 (CST); anti-AKT(phosphoThr308), 1:1000 (CST); anti-AKT, 1:1000 (CST); anti-Bcl-xL, 1:1000 (CST); anti-Bcl-2, 1:1000 (CST).

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