Briefly, 1 μg RNA was reversely transcribed into cDNA using ReverTra Ace (Toyobo, Osaka, Japan) with random hexamers. Primers (Table S5) were designed using Primer Premier 5.0 (Premier Biosoft, Palo Alto, USA). PCR was conducted in a 20 μL reaction system consisting of 10 μL 2 × SYBR Green PCR Master Mix (Toyobo), 200 nM primers, and 2 μL of 1:40-diluted cDNA using the DNA Engine Option 2 Real-Time PCR Detection System and Opticon Monitor software (Bio-Rad, USA). MaACT1 was selected as a housekeeping gene. The relative expressions of target genes were determined by the 2-△△Ct method [70]. The primers used for qRT-PCR were listed in Table S5.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.