Briefly, 1 μg RNA was reversely transcribed into cDNA using ReverTra Ace (Toyobo, Osaka, Japan) with random hexamers. Primers (Table S5) were designed using Primer Premier 5.0 (Premier Biosoft, Palo Alto, USA). PCR was conducted in a 20 μL reaction system consisting of 10 μL 2 × SYBR Green PCR Master Mix (Toyobo), 200 nM primers, and 2 μL of 1:40-diluted cDNA using the DNA Engine Option 2 Real-Time PCR Detection System and Opticon Monitor software (Bio-Rad, USA). MaACT1 was selected as a housekeeping gene. The relative expressions of target genes were determined by the 2-△△Ct method [70]. The primers used for qRT-PCR were listed in Table S5.

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