Transcriptional profiling of Cavendish banana WT and MaICE1-overexpressing plants (transgenic line #13) was conducted by RNA-Seq analysis at the Beijing Genomics Institution (BGI). Three biological replicates were adopted for each genotype under normal conditions and cold stress (10 °C for 1 and 4 h). RNA isolation, library construction, and sequencing on the BGISEQ-500 platform of PE 100 with 30 million reads per sample were performed at BGI (www.genomics.org.cn, BGI, Shenzhen, China). Gene expressions were determined using the RSEM software package [68]. To further confirm the reliability of those transcriptomic data, the expressions of four up-regulated genes were examined by qRT-PCR with specific primers (GSP7, GSP8, GSP9, and GSP10) as shown in Table S5. The DEGseq approach was employed to screen DEGs between groups with the criteria of a fold change ≥2 and adjusted p-value ≤0.001 as previously described [69]. Gene Ontology (GO) pathway annotation and enrichment analyses were carried out based on the GO database (http://www.geneontology.org/) and the KEGG pathway database (http://www.genome.jp/kegg/), respectively.

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