The first young leaf on the top of each of the three plants was collected at each time point (10 °C for 0, 3, 5, and 7 days and recovery after cold stress for 2 days) for each biological replicate. The MDA content was determined by the plant MDA assay kit (Nanjing Jiancheng Bio, Nanjing, China), and the POD activity was tested by the peroxidase assay kit (Nanjing Jiancheng Bio, Nanjing, China). Five replicates were performed for each sample. Three independent experiments were performed as biological replicates.

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