The expression level of each unigene was calculated by reads per kilobase millon mapped reads (RPKM) to assess the length and depth of sequencing [33]. Then, the differences in the expression abundance of each gene between each pair of compared samples were calculated by DESeq 2 software (version 1.4.5) [34]. Each resulting p-value was adjusted to a q-value, following the Benjamini-Hochberg procedure for controlling the false discovery rate [35]. The DEGs were identified with q ≤ 0.05 and |log2(fold-change)| ≥ 1 as thresholds. Then, the GO and KEGG analyses were considered to be significantly enriched with q ≤ 0.05 [36]. A GO functional enrichment analysis was performed using the BiNGO plugin of Cytoscape [37].

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