Tissue microarrays of clinical samples consisted of ESCC and paired normal adjacent tissues (NAT).One tissue microarray included 34 paired cases of ESCC and matched NAT (catalog number: # HEsoS180Su08; Outdo Biotech, Shanghai, China), and another 50 additional independent, subjected to esophagectomy, obtained from the First Affiliated Hospital of Xinjiang Medical University. Tumor tissues and clinicopathological parameters were collected after obtaining the informed consent from each participant involved. The study was approved by Medical Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University (Approval Number:2018K06–20).

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