The raw reads were filtered to remove adapter-polluted reads, low-quality reads, and reads with more than 5% ambiguous nucleotides. These clean reads with high-quality were subjected to the following analyses. Trinity software [30] was used to perform the de novo transcriptome assembly with default parameter values.

The assembled unigenes were annotated by homology search to publicly accessible databases using local BLAST programmes (version 2.2.28) with a significance threshold of E < 1e-5. Meanwhile, all unigenes were analysed with Blast2GO (version 3.0.8) to obtain the gene ontology (GO) annotations [31], which included BP, CC, and MF, with an E-value cut-off = 1e-5. Web Gene Ontology annotation software was adopted to perform GO functional classifications [32]. Furthermore, the sequences were searched against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database using KEGG Automatic Annotation Server (KAAS) with an E-value threshold <1e-10.

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