All of the animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Shanghai Bioduro Biologics Co., Ltd. Six- to eight-week-old female NOD/SCID/IL2Rγ−/− (NSG) mice were purchased from Shanghai Bioduro Biologics Co., Ltd. and raised under specific pathogen-free (SPF) conditions. The mice were euthanized after exhibiting moribund features, such as a large tumor burden, obvious weight loss, hunched posture, and lethargy. Xenograft tumors were established by the subcutaneous injection of 1 × 107 SKOV-3-luc cells in the presence of a 50% solution of Matrigel (BD Biosciences) in phosphate buffered saline (PBS). Eight days post inoculation, mice bearing established tumors were treated with intravenous injections of 5 × 106 meso3 CAR-T or meso3-CD40 CAR-T cells, and the bioluminescence was measured using serial imaging on a Xenogen IVIS Spectrum System (Life Technologies).

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