Cytokine release assays were performed by coculturing 1 × 106 CAR-T cells with immobilized rhMSLN antigen in six-well plates. After 24 h, the supernatants were assayed for the presence of all cytokines using the Cytometric Bead Array according to the manufacturer’s instructions (BD Biosciences). The values represent the mean of the triplicate wells.

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