CAL27 or HTB-43 cells were plated in 96-well plates. After 24 h, transfection with 25 nM of miR-34a-5p mimic or control mimic (Ambion) was performed with Lipofectamine RNAiMAX (Thermofisher). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was performed using the Vybrant® MTT Cell Proliferation Assay Kit, as described by the manufacturer. The absorbance of the samples was measured at 595 nm using a microtiter plate reader. Experiments were assayed in triplicate.

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