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The ten species used in this study were rhesus macaque (Macaca mulatta), common marmoset (Callithrix jacchus), mouse (C57BL/6 J, Mus musculus), rat (Brown Norway, Rattus norvegicus), rabbit (Oryctolagus cuniculus), cat (Felis catus), dog (Beagle, Canis familiaris), horse (Welsh Mountain Pony, Equus ferus), pig (domestic pig, Sus scrofa), and gray short-tailed opossum (Monodelphis domestica). All individuals used in this study were adults with no known health issues. Wherever possible, tissues from young adult males were used; however, some tissues were from females or older individuals. An overview of the origin, sex, and age for each animal used in the study is given in Additional file 1: Table S1. The details for each individual animal and tissue are given in Additional file 2: Table S2.

The use of all animals in this study was approved by the Animal Welfare and Ethics Review Board, under reference number NRWF-DO-02vs, and followed the Cancer Research UK Cambridge Institute guidelines for the use of animals in experimental studies. Tissues from seven species (macaque, marmoset, rabbit, cat, dog, horse, and opossum) were excess from routine euthanasia procedures (e.g., from individuals sacrificed during maintenance of research or breeding colonies). Tissues from three species (mouse, rat, and pig) were purchased commercially (e.g., from animal research supply companies.)

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