Plant materials and growth conditions
This protocol is extracted from research article:
Heritable gene editing using FT mobile guide RNAs and DNA viruses
Plant Methods, Feb 17, 2021; DOI: 10.1186/s13007-021-00719-4

A. thaliana Col-0 ecotype plants were transformed by the floral dipping method as previously described [22]. For the selection of transformants, seeds were sterilized with 5% NaClO for 5 min and plated on 1/2 MS medium [23] with 30 μg/mL kanamycin. After 2 weeks, resistant seedlings were transplanted to the soil under long-day conditions (16 h light/8 h dark) at 22 °C. Homozygous transgenic seedlings grown for about 15 days were used for inoculation transformation experiments.

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