Paraffin-embedded human-epithelial tissues, HNSCC tissues, and dysplastic oral squamous cell tissues were immunostained for MET protein using a laboratory established protocol. Briefly, deparaffinization, sequential ethanol treatment and antigen retrieval was performed. The process was followed by blocking and inactivating endogenous peroxidase with 3% H2O2, addition of the primary antibody (overnight; 4 °C) and addition of biotin-labeled secondary antibody (Room temperature; 30 mins). DAB was used for staining. Slides were digitally imaged at 20X magnification and analyzed within the Aperio Spectrum Database.

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