Medium IL-1β and IL-6 were measured using ELISA kits from R&D Systems (DY501-05 and DY506-05; Minneapolis, MN, USA) according to the manufacturer’s instructions. Goat serum was from Gibco (16210-064; Grand Island, NY, USA). Absorbance was read at 450 nm and 540 nm, respectively. The concentration of each sample was calculated from the standard curve prepared using the included standards.

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