Rat bone marrow mesenchymal stem cells (BMSCs) purchased from Cyagen Biotechnology Co., Ltd. (Suzhou, China) were maintained in a α-MEM medium supplemented with 10% fetal bovine serum (Gibco, 10091148, NY, USA) and 1% of an antibiotic-antimycotic solution (Sigma-Aldrich, A5955, USA) at 37 °C, 5% CO2, and 95% humidity. When it was necessary to induce osteogenic differentiation of BMSCs of passage 3, we replaced the α-MEM medium with the osteogenic medium (OM) which was prepared by adding 10 nM dexamethasone, 10 mM β-glycerophosphate, and 50 mg/ml ascorbic acid to the α-MEM complete medium. The culture medium was refreshed every 3 days.

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