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Characterization of PCL/nHA scaffolds
This protocol is extracted from research article:
Low-frequency electromagnetic fields combined with tissue engineering techniques accelerate intervertebral fusion
Stem Cell Res Ther, Feb 17, 2021;

Procedure

The porosity of PCL/nHA composite scaffolds was measured as described before [27]. Briefly, apparent volume (Va) and dry weight (Wd) of the porous scaffolds (n = 6) were measured before they were immersed in 95% ethanol for 5 min. The scaffolds were washed with distilled water and kept in distilled water overnight to determine the wet weight (Ww) of the scaffolds. p indicated the density of the distilled water and the scaffold porosity was calculated as: porosity (%) = $ww−wdpva.$

Instron 5566 device (Instron Corporation, USA) was utilized to evaluate the mechanical properties of scaffolds. Concisely, cubic specimens (n = 6) were placed vertically between two solid platens. The compression rate was set as 1 mm/min with a 5-N load cell. And the compression strength was acquired from the stress-strain curve.

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