HUVECs were allocated into seven different groups as follows; the non-treated Control cells; Pal; DHA, DHA+ Pal; Insulin, Insulin +Pal, and Insulin + DHA + Pal. To induce atherosclerotic changes, cells were treated with a culture medium containing 1 mM Pal (Cat no. P0500-10G, Sigma) and 2% FBS for 48 h. We also incubated HUVECs with 50 μM insulin for the induction of hyperinsulinemia conditions for 48 h. After completion of hyperinsulinemia condition and atherosclerotic changes, we added the 50μMDHA (Cat no. D253425MG, Sigma) to the respective groups and cells allowed to expose to DHA for 48 h.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.