The most commonly used method in the measurement of neurotransmitters is high-performance liquid chromatography (HPLC). The development of HPLC is due to the introduction of the gas chromatography theory on the basis of classic liquid chromatography. In previous studies on the biological effects of microwave radiation, HPLC was widely used to measure the level of neurotransmitters such as aspartic acid (Asp), glutamate (Glu), glycine (Gly), and gamma-aminobutyric acid (GABA) [3, 12, 36, 67, 68, 73, 92, 124, 228].

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