To evaluate the treatment protocol, we selected human umbilical vein endothelial cells (HUVECs). HUVECs were purchased from the National Cell Bank of Iran (NCBI code: C554). The cells were expanded in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12; Gibco) under reasonable condition consisted of 37 °C temperature, 5% CO2, and 95–98% humidity. To induce cell proliferation, culture media is supplemented with 10 10% fetal bovine serum (FBS) and 1% Pen-Strep solution. Cells were detached using 0.25% Trypsin-EDTA solution (Gibco). We enrolled the cells to different analyses between passages 3–6.

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