Endometrial adenocarcinoma HEC-1A and HEC-1B cell lines (ATCC® HTB-112 and ATCC® HTB-113 respectively; www.lgcstandards-atcc.org) [52] were maintained (37oC, 5 % CO2) in DMEM/F-12 + Glutamax™ full media (Gibco, Thermo Fisher, UK) supplemented with 10 % (v/v) foetal bovine serum (FBS), sodium bicarbonate 1mM, sodium pyruvate 1mM and 1 % (v/v) antibiotic-antimycotic solution in plastic culture vessels (25 cm2, 75 cm2, 125 cm2). Cells were supplemented with full serum media every two days and passaged when confluent. Only cells passaged more than two times were used in this study.

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