HCC cells were cultured in 10 cm dish with serum-free medium for 24 h. Marigel (Corning) was even plated onto the upper chamber of a Transwell insert (Corning, NY, USA). 5 × 104 HCC cells were suspended with 100 μL of serum-free medium and seeded into the top chamber. The lower chamber was incubated with serum-contained medium. 24 h later, the inserts were fixed with 4% paraformaldehyde (PFA) for 30 min. Cells on the top surface of the inserts were removed and migrated cells on the lower surface of the inserts were stained with 0.05% crystal violet. Images of cells on the Transwell membrane were taken with a microscope and cell numbers were counted. Data were shown as mean ± SD of three independent experiments.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.