The cDNAs of RNF152, FoxO1, FoxO3, FoxO4 and FoxO6 were amplified from 293 T or HepG2 cells by polymerase chain reaction (PCR) and cloned into pbabe-Flag vector. All plasmids were completely sequenced and transfected into cells by Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions.

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