At selected PDs throughout their proliferative lifespans, DPSCs expanded with or without H2O2 (0–200 μM) were maintained in 6-well plates as above, until 80–90% confluent. Following DNA purification [12], telomere length analyses were performed using the TeloTAGGG Telomere Restriction Fragment (TRF) Length Assay Kit (Roche, Welwyn Garden City, UK), per the manufacturer’s instructions. A digoxigenin (DIG)-labeled molecular weight marker (kb, in Kit) and positive DIG-labeled control DNA sample (CTRL, in Kit) were also included. Mean telomere lengths were calculated from Southern blot images via ImageJ® Software [12].

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.