Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues using standard procedures. Xenograft tissues were subjected to antigen retrieval by pressure cooking (citrate buffer, pH 6, 20 min) and 0.3% H2O2 endogenous peroxidase blocking. For primary antibody: TSP-1 monoclonal antibody (1:1000; Abcam), CD31 monoclonal antibody (1:500; Millipore), Ki-67 (1:150; Dako, Agilent Technologies), were incubated overnight at 4°C, treated with bio-tinylated secondary IgG antibodies for 30 min using ABC reagent kit and DAB chromagen (Vector Laboratories). A final counterstain was performed in hematoxylin followed by serial dehydration in ethanol and xylene and mounted in Permount (Thermo Fisher Scientific). Hematoxylin and eosin (H&E) stains were performed using standard protocols.

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