The seed genes in modules with the most connectivities referred to hub genes and TCGA KIRC data was used to perform validation using GEPIA database [24]. The RNA-sequencing (RNA-seq) data for hub genes were downloaded from the The Cancer Genome Atlas (TCGA, database. The analysis for expression level of hub genes between normal esophageal samples (n = 182) and ESCC samples (n = 286) was based on GTEx data in GEPIA from TCGA. Oncomine database was used to further analyse the expression level of hub genes with clinical traits [25, 26]. Logrank value p < 0.05 was considered to be statistically significant.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.