Human group 3 medulloblastoma established cell lines D283, D458, D458-TSP-1 (American Type Culture Collection) were maintained in zinc option medium (Life Technologies); and UW426-MYC were maintained in αMEM containing 10% fetal bovine serum (FBS). MP mouse tumor cells (human MYC-T58A & Trp53DN- infected neural stem cells; from Dr. Wechsler-Reya, Sanford-Burnham Medical Research Institute) were maintained in Neurocult complete medium with FGF1, EGF, and N2 supplements (Stem Cell Technologies) as described previously.7 G3 mouse MB cells (human MYC-infected Trp53-/-; Cdkn2c-/- GNPs) and Sonic Hedgehog (SHH) mouse MB (Ptch1+/- Trp53-/- GNPs) from Dr. Roussel, St. Jude Children’s Research Hospital, TN, were maintained as neurospheres in neurobasal medium with FGF1, EGF, B27 and N2 supplements as previously reported.6

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