Human group 3 medulloblastoma established cell lines D283, D458, D458-TSP-1 (American Type Culture Collection) were maintained in zinc option medium (Life Technologies); and UW426-MYC were maintained in αMEM containing 10% fetal bovine serum (FBS). MP mouse tumor cells (human MYC-T58A & Trp53DN- infected neural stem cells; from Dr. Wechsler-Reya, Sanford-Burnham Medical Research Institute) were maintained in Neurocult complete medium with FGF1, EGF, and N2 supplements (Stem Cell Technologies) as described previously.7 G3 mouse MB cells (human MYC-infected Trp53-/-; Cdkn2c-/- GNPs) and Sonic Hedgehog (SHH) mouse MB (Ptch1+/- Trp53-/- GNPs) from Dr. Roussel, St. Jude Children’s Research Hospital, TN, were maintained as neurospheres in neurobasal medium with FGF1, EGF, B27 and N2 supplements as previously reported.6

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.