Wilcoxon rank-sum tests were performed to detect differences in the skin physiological and microbial characteristics between the three facial sites, including clinical parameters, gene count, Shannon index, and the relative abundances of species, KOs, and modules. For a certain phenotype feature (male/female), Fisher’s exact test was used. Unless otherwise indicated, P values were adjusted using the FDR correction by fdrtool package [74] in R. Statistical significance was set as adjusted P value < 0.05. Differentially enriched KEGG modules and KOs were identified, according to FDR adjusted P values. We used Wilcoxon rank-sum tests to obtain P values. FDR adjusted P values of less than 0.05 was used as the detection threshold for significance.

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