Multivariate statistical analyses (PCA, PCOA) were applied to assess the skin microbiome within individuals. Principle component analysis (PCA) was performed on the three facial sites as previously described, using the ade4 package [73] in the R platform. Principle coordination analysis (PCOA) was performed based on the Jensen-Shannon distance (JSD)/Bray Curtis distance on the skin microbial composition and functional profile using the ade4 package [73].

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