To evaluate the genome integrity of a single microbe in iHSMGC, we constructed draft microbial reference genomes of 5409 bacteria, 2023 viruses, and 158 fungi ( and sequenced alignment iHSMGC with the database. The definite means were as follows: (1) predicting the coding sequence (CDS) of genomes and (2) map iHSMGC with genome CDS using the BWA MEM method (default parameter). The coverage of each genomic CDS region was obtained.

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