To evaluate the genome integrity of a single microbe in iHSMGC, we constructed draft microbial reference genomes of 5409 bacteria, 2023 viruses, and 158 fungi (https://ftp.ncbi.nlm.nih.gov/genomes/) and sequenced alignment iHSMGC with the database. The definite means were as follows: (1) predicting the coding sequence (CDS) of genomes and (2) map iHSMGC with genome CDS using the BWA MEM method (default parameter). The coverage of each genomic CDS region was obtained.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.