Transfected NSCLC cells were seeded into 6-well plates (1 × 103 / well) and irradiated with the indicated single dose (0, 2, 4, 6 or 8 Gy). After 2 weeks of culture, cells were fixed with 100% methanol and stained with 0.1% crystal violet for 15 min. The number of colony in each group was then counted and recorded with naked eyes.

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