H838 and A549 cells in the logarithmic growth phase were selected and then trypsinized. In brief, 100 μL cell suspension containing 2 × 103 cells was added into each well of the 96-well plates. Then the 96-well plates were placed in an incubator to continue the culture. After 24 h, 10 μL of cell counting kit 8 (CCK-8) solution (Biossci, Wuhan, China) was added to each well and incubated for another hour in the incubator. After the end of the culture, the 96-well plates were placed in a microplate reader, and the absorbance (optical density, OD value) of each well at a wavelength of 450 nm was measured. Thereafter, the absorbance of the cells was measured at 48, 72, and 96 h, respectively.

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