Percoll‐isolated monocytes (10 × 106) were seeded into Petri dishes (Greiner) and stimulated in 10 mL culture medium for 24 h, washed with warm PBS and incubated in normal culture medium at 37°C, 5% CO2. At day 6, cells were detached with Versene solution (Thermo Fisher Scientific) and 1 × 105 cells were plated in quintuplicate to overnight‐calibrated cartridges in assay medium [RPMI with 2 mmol L−1 glutamine, 11 mmol L−1 glucose and 1 mmol L−1 pyruvate (pH adjusted to 7.4)] and incubated for 1 h in a non‐CO2 incubator at 37°C. OCR and ECAR were measured using a Cell Mito Stress Kit in an XFe96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent Technologies, Santa Clara, CA, USA), with final concentrations of 1 μmol L−1 oligomycin A (Sigma), 1 μmol L−1 carbonyl cyanide‐4‐(trifluoromethoxy) phenylhydrazone (FCCP, Sigma), and 0.5 μmol L−1 rotenone (Sigma)/antimycin A (from Streptomyces sp., Sigma). The protocol was followed using the manufacturer's descriptions and compounds.

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