The cell proliferation of 32D cells was determined by the CCK-8 assay kit (Beyotime, Beijing, China). The 32D cells with different treatments were seeded onto the 96-well plate at a density of 2,000 cells/well. After culturing for indicated time duration, the cells were incubated with 10 µL of CCK-8 well at 37 °C for 3 h. The proliferation of 32D cells was determined by measuring absorbance at 450 nm.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.