Chromatin immunoprecipitation of healthy individuals was performed of Percoll‐isolated monocytes at day 6 of the in vitro training protocol. Percoll‐isolated monocytes of bladder cancer patients were immediately processed at the day of blood drawing. Cells were cross‐linked with methanol‐free 1% formaldehyde, sonicated and immunoprecipitated using 1 µg of H3K9me2 antibody (Diagenode, Liège, Belgium). Immunoprecipitated chromatin was processed further for qRT‐PCR analysis using the MiniElute DNA purification kit (Qiagen). Primers used in the reaction are listed in Supplementary table 6. Samples were analysed with a comparative Ct method on the StepOne PLUS qPCR machine (Thermo Fisher Scientific) using SYBR green (Invitrogen) in accordance with the manufacturer's instructions.

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