Quantitative RT‐PCR of in vitro trained monocytes was performed by isolating RNA using TRIzol reagent according to the manufacturer's instructions. cDNA was synthesised with the SuperScript III First‐Strand Synthesis System (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer's protocol. Quantitative RT‐PCR was performed using StepOne PLUS machine (Thermo Fisher Scientific) with SYBR Green (Invitrogen, Waltham, MA, USA). The values are expressed as log2 fold increase in mRNA levels relative to those in non‐trained cells. 18S was used as a housekeeping gene. The primer sequences are listed in Supplementary table 5.

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