The 32D cell line was a generous gift from the Toledo University (Ohio, USA). The 32D cells were cultured with RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10 ng/ml of interleukin-3 (Sigma, St. Louis, MO, USA) and 10% fetal bovine serum (Thermo Fisher Scientific) and were kept in a humidified incubator with 5% CO2 at 37°C. The cell culture medium was changed every 3–4 days.

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