Cells were isolated by density centrifugation on Ficoll‐Paque (GE Healthcare, Chalfont St Giles, UK), washed three times in PBS and resuspended in culture medium. After isolation, 5 × 105 PBMCs were added to a round bottom 96‐well plate (Greiner, Kremsmünster, Austria). Pam3Cys (10 μg mL−1) or LPS (10 ng mL−1) was added for 24 h at 37°C and 5% CO2.

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