To assess the adhesion ability of HUVECs, a cell adhesion assay was performed with slight modifications according to WOOD [30]. Briefly, the 96-well plates were coated with conditioned medium (50 μl/well) overnight in a 5% CO2 incubator at 37 °C. The next day, the CM was removed, and the 96-well plates were air-dried in the biosafety cabinet. Then, HUVECs (0.5 × 103 cells/well) were suspended in 100 μl of conditioned medium and seeded into the corresponding CM-coated 96-well plates. After being incubated for 2 h, the cells were washed to remove nonadherent cells. Adherent cells were fixed with 4% paraformaldehyde and stained with 0.1% gentian violet. Three to five visual fields per well were randomly selected to take pictures and count cells.

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