All statistical analyses were performed using SPSS software ver. 26.0 (IBM Corp., Armonk, NY, United States). Extreme outliers were excluded prior to statistical analysis using the boxplot tool in SPSS (more than 3x the interquartile range outside of the end of the interquartile box). Molecular and behavioral data were analyzed with two-way analysis of variance (ANOVAs) for main effects of LPS exposure, diet, or interaction between the two factors (LPS/saline * standard/VTX-enriched diet). When a significant main effect was found, planned pairwise comparisons were performed by one-way ANOVA followed by Tukey’s HDS post hoc test (LPS vs. saline animals receiving the same diet for 4 weeks or standard vs. VTX-enriched diet in mice injected with either LPS or saline). Analysis of NORTs involved one-sample t-tests comparing each group’s mean percent preference with a hypothetical mean of 50% chance preference (Keppel 1991). A p level of <0.05 was regarded as significant for all tests.

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