Assessment of serum corticosterone levels was done by means of enzyme immunoassay (EIA) using a commercially available kit (Arbor Assays, Ann Arbor, MI, United States), which utilizes a microplate reader set at 450 nm, following the manufacturer’s instructions. Serum samples were diluted 1:150 in appropriate assay buffer and assayed in duplicate. The detection limit of the assay was 16.9 pg/ml; intra- and interassay coefficients of variations were 8.15% and 17.93%, respectively.

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