Trunk blood was collected after decapitation for the determination of glucocorticoid levels as an indication of HPA activation. Experiments were performed from 9:00 am to 4:00 pm during the light period. To improve serum separation from whole blood, samples were let to clot 15 min at room temperature and 1 h on ice before centrifugation (1,000 × g for 15 min). Serum was transferred into clean tubes and stored at −80 °C until the assay.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.