Adult C57BL/6J male mice (n = 87; Charles River Laboratories, Lecco, Italy) between 11 and 16 weeks of age were used in all experiments. Mice were habituated for 5 weeks prior to pharmacological treatment and group-housed (four per cage) in polycarbonate cages (30 × 30 × 15 cm) with ad libitum access to food and water throughout the study while being maintained under a 12/12 h light-dark cycle (lights on 8:00 am to 8:00 pm), in an ambient temperature of 21 ± 3 °C with relative controlled humidity. Animals were checked daily for signs of discomfort as indicated by the animal care and use guidelines [National Academy of Sciences. Guide for the Care and Use of Laboratory Animals, 1998, “Guidelines for the Care and Use of Mammals in Neuroscience and Behavioral Research” (National Research Council 2003)]. All procedures were carried out in accordance with the EC guidelines (EEC Council Directive 86/609 1987) and the Italian legislation on animal experimentation (Decreto Legislativo 26/2014) and had the approval of the local Ethical Committee. Mice were handled twice a week throughout the experiment to habituate the animals to the experimenters.

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