The entire otc gene cluster located on the pYAC-ΦC31-Ts-OTC plasmid was transformed into E. coli ET12567/pUB307. In the next step, the plasmid was conjugated into ATCC 10970 ΔOTC, 15883S and HP0508 ΔOTC, via E. coli ET12567/pUB307–S. rimosus conjugation [39]. As a control, pYAC-ΦC31-Ts-h without the otc gene cluster was introduced into all three otc-deletion S. rimosus strains. Conjugants were patched on MS selective medium and cultured and analyzed as described in Sects. Media and culture conditions" and "High performance liquid chromatography".

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