SGC-7901 or MKN-45 cells were plated in 24-well plates, fixed, permeabilized, blocked, and incubated overnight at 4 °C with anti-NF-κB p65 (Cell Signaling Technology, 8242, 1:100). They were then rinsed and probed using AF594-linked goat anti-rabbit IgG (ABclonal, Wuhan, China; AS039). DAPI was then used for nuclear counterstaining, and cells were imaged via a BX41 microscope (Olympus).

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