All statistical analyses were compiled using GraphPad Prism for windows version 8.2.1 (GraphPad Software, Inc., La Jolla, CA, USA). For all analyses, the experimental unit was individual samples obtained from a minimum of 4 (n = 4) groups were assessed. One passage of cells was equivalent to one group of samples (n). The effect of multiple testing was controlled for by measuring the statistical significance of each association using both the P-value and the q-value. Using FDR of q < 0.05, the q value quantifies significance in terms of the false discovery rate (FDR) rather than the false positive rate and forms a measure of how likely a particular P-value is to represent a genuine association. For all analyses, significance was set at P 0.05 where data are expressed as mean ± SEM based on t-test for pairwise analysis and/or ANOVA analysis (with Kruskal-Wallis post hoc test).

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