Lipid content was quantified by determining the level of inorganic phosphorus using the Bartlett Assay [16]. Sulfuric acid 400 μL (5 M) was added to lipid extracts (10 μL) in a glass test tube and heated at 180–200 °C for 1 h. H2O2 (100 μL of 30% v/v) was then added while vortexing and the extract heated at 180–200 °C for 1.5 h. Reagent (4.6 mL of 1.1 g ammonium molybdate tetrahydrate in 12.5 mL sulfuric acid and 500 mL ddH2O) was added followed by vortexing. Then, 100 μL of 15% ascorbic acid (v/v) was added, followed by further vortexing. The solution was then heated for 7–10 min at 100 °C. A 150 μL aliquot was used to measure the absorbance at 830 nm.

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